江苏省科技期刊可视化阅读平台

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江苏科技信息 ›› 2015, Vol. 32 ›› Issue (24): 47-48.doi: 10.3969/j.issn.1004-7530.2015.24.013

• 论文 • 上一篇    下一篇

阿魏酸钠对抗结核药物致大鼠肝损伤的保护作用及其机制研究

文高艳, 曾谊   

  1. 南京市胸科医院 东南大学医学院附属南京胸科医院,江苏南京,210000
  • 出版日期:2015-08-25 发布日期:2015-08-25

Study on the Mechanism and Protective Effects of Sodium Ferulate on the Hepatic Injury Induced by Antituberculosis Drugs in Rats

Wen Gaoyan, Zeng Yi   

  • Online:2015-08-25 Published:2015-08-25

摘要： 为探讨阿魏酸钠（SF）对抗结核药物肝损伤的保护作用机制，文章将60只SD雄性大鼠随机分为6组，即对照组、模型组（异烟肼+利福平）、SF低、中、高剂量组（30g/kg，60g/kg，120g/kg）和硫普罗宁组（60 mg/kg），给药8周。分别测定大鼠血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、白介素-1β（IL-1β）、IL-6和肿瘤坏死因子-α（TNF-α）水平。采用Western Blotting方法测定大鼠肝脏TNF-α和核转录因子-κB（NF-κB）蛋白表达水平。与模型组比较，SF中高剂量组均显著降低血清ALT，AST，IL-1β，IL-6和TNF-α水平，并降低肝脏TNF-α和NF-κB蛋白表达水平。结果证明，SF对抗结核药物肝损伤的保护作用机制可能与抑制NF-κB表达有关。

关键词: 抗结核药, 肝损伤, TNF-α, NF-κB

Abstract: In order to investigate the protective effects of sodium ferulate (SF) on the hepatic injury induced by antituberculosis drugs in rats,the paper divided randomly sixty male SD rats into 6 groups:normal group,model group (isoniazid and rifampin),SF (30g/kg,60g/kg,120g/kg)and tiopronin group (60mg/kg).One hour after the final treatment,the alanine aminotransferase (ALT),aspartate aminotransferase (AST),interleukin-1β (IL-1β),IL-6, tumor necrosis factor-α (TNF-α)levels in serum were measured.Expression of TNF-α and nuclear transcription factor-κB (NF-κB)protein levels were assayed by Western Blotting method.Compared to vehicle-model group,SF at medium and high doses significantly reduced serum ALT, AST,IL-1β,IL-6 and TNF-α levels,and down-regulated liver TNF-α and NF-κB protein levels.The results showed that the protective effects of SF on the hepatic injury induced by antituberculosis drugs in rats may be related to its inhibition of NF-κB.