江苏省科技期刊可视化阅读平台

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江苏科技信息 ›› 2015, Vol. 32 ›› Issue (21): 57-58.doi: 10.3969/j.issn.1004-7530.2015.21.026

• 论文 • 上一篇    下一篇

当归芍药散对药物性肝损伤小鼠的保护作用及其机制研究

孙旭, 唐宁   

  1. 中国食品药品检定研究院国家药物安全评价监测中心,北京,100050;江苏省泗阳县农业委员会,江苏宿迁,223700
  • 出版日期:2015-07-25 发布日期:2015-07-25

Protective Effect of Danggui Shaoyao San on Drug-Induced Liver Injury in Mice and Its Mechanism

Sun Xu, Tang Ning   

  • Online:2015-07-25 Published:2015-07-25

摘要： 观察当归芍药散对异烟肼（INH）和利福平（RFP）合用致小鼠药物性肝损伤的保护作用及其机制。将小鼠随机分为正常组、模型组、当归芍药散高、中、低剂量组（32，16，8mg/kg）和联苯双酯组（200mg/kg）。采用INH和RFP合用复制小鼠药物性肝损伤模型，测定血清中谷丙转氨酶（ALT）、谷草转氨酶（AST），同时测定肝匀浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）含量；HE染色法观察肝组织病理学变化，分析当归芍药散的保肝作用及其可能机制。结果表明当归芍药散可降低INH和RFP联用所致药物性肝损伤小鼠肝脏系数，显著降低小鼠血清中ALT（P<0.01），降低AST（P<0.05）含量；肝脏MDA含量明显降低（P<0.05），SOD活力显著提高（P<0.05），GSH-Px含量呈增高的趋势；病理学检查表明当归芍药散能显著减轻肝损伤程度。文章指出当归芍药散对药物性肝损伤小鼠具有一定的保护作用，其机制可能与其抗氧化作用有关。

关键词: 当归芍药散, 药物性肝损伤, 抗氧化, 异烟肼, 利福平

Abstract: To investigate the protective effect and mechanismof saponins of Danggui Shaoyao San(DSS)on drug-induced liver injury in mice. Method mice were randomly divided into six groups,control group,low,middle,high-dose (8,16,32mg/kg)of DSS,model group and bifendate group(200mg/kg).The model of drug-induced liver injury was induced by intragastric administrationof isoniazid(INH) and rifampin(RFP) in mice.The levels of alanine aminotransferase(ALT),aspartate transaminase(AST)in serum and the content of superoxide dismutase(SOD), malondialdehyde(MDA),glutathione peroxidase(GSH-Px)inliver homogenate were assayed by spectrophotometry. The pathological changesof hepatic tissueswere observed by hematoxylin and eosin(HE)staining.Result The liver index DSS middle,high-dose groups was lower than the model group(P<0.05 or P<0.01).Compared with the model group,the levels of ALT and AST In DSS middle,high-dose groups were significantly decreased( P<0.05 or P<0.01). The content of MDA DSS middle,high-dose groups was also lower than that in the model group(P<0.05 or P<0.01), while the activity of SOD and GSH-Px in DSS middle,high-dose groups was higher than the model group(P<0.05 or P<0.01).Hepatic tissues stained with HE showed that PNS could remark ablyre lieve liver injury.The article points out DSS has protective effect on drug-induced liver injury in mice,which may be related toantioxidant effect.