江苏省科技期刊可视化阅读平台

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江苏科技信息 ›› 2015, Vol. 32 ›› Issue (23): 30-31.doi: 10.3969/j.issn.1004-7530.2015.23.023

• 论文 • 上一篇    下一篇

间接ELISA法检测蚕豆花粉过敏原最优条件的建立

王楠   

  1. 盐城卫生职业技术学院,江苏盐城,224005
  • 出版日期:2015-08-15 发布日期:2015-08-15

Establishment of Indirect ELISA Techniques to Detect Broad Bean Pollen Allergen

Wang Nan   

  • Online:2015-08-15 Published:2015-08-15

摘要： 油菜花粉过敏原粗提液为抗原包被酶标板，文章通过对间接ELISA各反应条件的优化，确定了最佳反应条件。结果血清稀释度为1∶10，anti-human IgE/HRP稀释度为1∶1000。采用已建立的间接ELISA反应条件，对10份阴性血清的检测结果进行统计学分析，确定间接ELISA的判定标准，D450值大于等于0.158为阳性。

关键词: 间接ELIS法, 蚕豆花粉过敏原, 最优条件

Abstract: By optimizing the reaction conditions for each indirect ELISA ,the paper determines the optimal reaction conditions.With oilseed rape pollen allergen as a capture antigen ,the proper dilution of anti-human IgE/HRP was 1∶1000 and the proper dilution of serum was 1∶10.The cut-off value of the assay was determined by statistics analysis based on 10 negative samples,with D450 equal to or higher than 0.158 being considered positive.