江苏科技信息 ›› 2015, Vol. 32 ›› Issue (23): 30-31.doi: 10.3969/j.issn.1004-7530.2015.23.023
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王楠
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Wang Nan
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摘要: 油菜花粉过敏原粗提液为抗原包被酶标板,文章通过对间接ELISA各反应条件的优化,确定了最佳反应条件。结果血清稀释度为1∶10,anti-human IgE/HRP稀释度为1∶1000。采用已建立的间接ELISA反应条件,对10份阴性血清的检测结果进行统计学分析,确定间接ELISA的判定标准,D450值大于等于0.158为阳性。
关键词: 间接ELIS法, 蚕豆花粉过敏原, 最优条件
Abstract: By optimizing the reaction conditions for each indirect ELISA ,the paper determines the optimal reaction conditions.With oilseed rape pollen allergen as a capture antigen ,the proper dilution of anti-human IgE/HRP was 1∶1000 and the proper dilution of serum was 1∶10.The cut-off value of the assay was determined by statistics analysis based on 10 negative samples,with D450 equal to or higher than 0.158 being considered positive.
王楠. 间接ELISA法检测蚕豆花粉过敏原最优条件的建立[J]. 江苏科技信息, 2015, 32(23): 30-31.
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链接本文: https://qkcb.jssti.cn/jskj/CN/10.3969/j.issn.1004-7530.2015.23.023
https://qkcb.jssti.cn/jskj/CN/Y2015/V32/I23/30